Search results for "fusion proteins"
Article
Fusion Proteins Pose Manufacturability Challenges
Challener
Fusion proteins are engineered proteins that contain domains from different proteins that originate from different sources, such as a cell surface receptor or cytokine (i.e., interferon …
Article
Optimization of Protein Expression in Escherichia Coli
May 01, 2015
By Siavash Bashiri, David Vikström, Nurzian Ismail
BioPharm International
Volume 28, Issue 5
Production of proteins for manufacturing therapeutics and pharmaceuticals is a c…
Article
Re-use of Protein A Resin: Fouling and Economics
Maximizing the lifespan of preparative Protein A columns is a central topic during process development for monoclonal antibodies and Fc fusion proteins. It is clear that the industry does not have a d…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Examples of the purification challenges involved with two Fc-fusion proteins will be described in the following sections.
Because each Fc-fusion protein has a different protein sequence fused to a…
Article
Host-Cell Protein Measurement and Control
Jun 01, 2015
By Fengqiang Wang, PhD, Daisy Richardson, Mohammed Shameem
BioPharm International
Volume 28, Issue 6, pg 32–38
Host-cell proteins (HCPs) constitute a major part of proce…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
This early screening approach has identified cryptic aberrant splice sites in multiple therapeutic candidates including monoclonal antibodies, bispecific antibodies, and fusion proteins, therefore pre…
Article
Best Practices for Selecting a Top-Quality Cell Line
“Quickly developing and delivering high-quality cell lines is getting more and more challenging for bispecific antibodies and fusion proteins. It is difficult to rapidly screen more cell types clones …
Article
Quality by design for biotechnology products—part 1
A PhRMA Working Group's advice on applying QbD to biotech.
By Taruna Arora, Roger Greene, Jennifer Mercer, Paul Tsang, Meg Casais, Stuart Feldman, Jutta Look, Tony Lubiniecki, Joseph Mezzatesta,…
Article
Platform Technologies Improve Protein Expression
Recombinant proteins, peptides, multi-subunit molecules, monoclonal antibodies, fusion proteins, and enzymes are manufactured within a growing plant using sunlight, soil, water, and air as raw materia…
Article
A Look at the Affinity Chromatography Landscape
They have also needed to develop resin chemistries more suitable for the growing range of protein-based biologics, such as fusion proteins and antibody fragments, and affinity ligands that can separat…